thr 77 - An Overview
thr 77 - An Overview
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Antibodies certain to the blocking peptide no longer bind into the epitope about the goal protein. By evaluating the staining in the blocked antibody compared to the antibody by itself, one can see which staining is specific.
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This information outlines further more achievable optimization of mobile and immunoassay parameters to make certain the very best final results are...
This guideline outlines additional doable optimization of mobile and immunoassay parameters to make certain the very best success are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind precisely towards the target antibody and block antibody binding. These peptide ordinarily contains the epitope regarded via the antibody.
Concentrate on Information and facts Cyclin E belongs on the hugely conserved cyclin household, whose members are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins show unique expression and degradation styles which add on the temporal coordination of each mitotic celebration. Cyclin E varieties a posh with and features like a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by way of S stage.
Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was click here observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential function in endorsing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two further splice variants were being noted but specific nucleotide sequence info will not be yet offered.
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